anti mouse cd40 (Bio X Cell)
Bio X Cell is a verified supplier 
Bio X Cell manufactures this product 
96
Structured Review
Bio X Cell
anti mouse cd40
Anti Mouse Cd40, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mouse+cd40/InVivoMAb+anti-mouse+CD40/pmc13041753-259-7-11
Average 96 stars, based on 204 article reviews
Anti Mouse Cd40, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mouse+cd40/InVivoMAb+anti-mouse+CD40/pmc13041753-259-7-11
Average 96 stars, based on 204 article reviews
anti mouse cd40 - by Bioz Stars,
2026-10
96/100 stars
Images
1) Product Images from "Inflammatory arthritis irAE may represent a unique autoimmune disease primarily driven by T cells but likely not autoantibodies"
Article Title: Inflammatory arthritis irAE may represent a unique autoimmune disease primarily driven by T cells but likely not autoantibodies
Journal: Science Advances
doi: 10.1126/sciadv.aea4262
Figure Legend Snippet: ( A to C ) Human naïve B cells were isolated from PBMCs and cultured under indicated conditions for 7 days; pembrolizumab or isotype control IgG4 (IgG4 Iso) was added on day 2; n = 8. (A) Expression of CD38 and CD27 on B cells. Right: Percentages of CD27 + CD38 − and CD27 + CD38 + B cells. (B) Expression of CD138 on CD27 + CD38 + B cells. Right: Percentage of CD138 + CD27 + CD38 + B cells. (C) Different immunoglobulin isotypes were measured in the culture supernatant of (A) and (B) by multiplex assay. ( D to F ) B cells from Humanized PD-1 (HuPD-1) mice were isolated and cultured with LPS, IL-4, BAFF, or ODN 2006, anti-IgM, IL-21, IL-4 or R848, anti-IgM, anti-CD40, IL-21, and IFN-γ for 3 days; pembrolizumab or isotype control was added on day 1. (D) Expression of IgG2c on activated B cells. FSC-H, Forward scatter height. Right: Percentage of IgG2c + B cells; n = 5. (E) Expression of IgG1 on activated B cells. Right: Percentage of IgG1 + B cells; n = 5. (F) Different immunoglobulin isotypes in the supernatant were measured by multiplex assay; n = 5. Data in graphs represent mean ± SEM. Significance was tested by two-way ANOVA.
Techniques Used: Isolation, Cell Culture, Control, Expressing, Multiplex Assay
![( A to F ) Bead-based multiplex assays were used to measure plasma concentration of IL-6, and IL-12p70 (A); TNF-α, IFN-γ, and IL-1β [B; HC ( n = 19), irAE ( n = 34), RAC ( n = 45), and ICI ( n = 9)]; IP-10 (CXCL10), CXCL11, and CXCL9 (C; HC, n = 17; irAE, n = 33; RAC, n = 46; ICI, n = 17); CCL20 (D); CX3CL1 (E); and CCL2 (F). ( G to J ) Human naïve B cells were isolated and cultured with anti–human CD40 (0.5 μg/ml), anti–human Ig (M + G + A) (2.5 μg/ml), and rhIL-21 (20 ng/ml) with IFN-α (100 ng/ml), IL-6 (100 ng/ml), IL-12 (100 ng/ml), control, or the combination of IFN-α, IL-6, and IL-12 for 7 days. Cells and culture supernatants were analyzed. (G) Representative flow plot of CD38 and CD138 expression on CD27 hi CD38 hi ASCs. Right: A summary of the percentage of CD138 + ASCs; n = 6. (H) Expression of CD11c and CD27 on CD27 − IgD − B cells. Right: Percentage of CD11c + IgD − CD27 − B cells; n = 6. (I) Expression of active-caspase-3 in B cells. Right: Percentage of active-caspase-3 + B cells from different groups; n = 3. (J) Different immunoglobulin isotype levels in the culture supernatants from (G) to (H) were measured by the multiplex assay; n = 6. Data in graphs represent mean ± SEM. Significance was tested by one-way ANOVA [(A) to (I)] and paired Student’s t test (J). [(A) to (F)] ICI, ICI control.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1753/pmc13041753/pmc13041753__sciadv.aea4262-f5.jpg "... B cells were isolated and cultured with anti–human CD40 (0.5 μg/ml), anti–human Ig (M + G + ...")
Figure Legend Snippet: ( A to F ) Bead-based multiplex assays were used to measure plasma concentration of IL-6, and IL-12p70 (A); TNF-α, IFN-γ, and IL-1β [B; HC ( n = 19), irAE ( n = 34), RAC ( n = 45), and ICI ( n = 9)]; IP-10 (CXCL10), CXCL11, and CXCL9 (C; HC, n = 17; irAE, n = 33; RAC, n = 46; ICI, n = 17); CCL20 (D); CX3CL1 (E); and CCL2 (F). ( G to J ) Human naïve B cells were isolated and cultured with anti–human CD40 (0.5 μg/ml), anti–human Ig (M + G + A) (2.5 μg/ml), and rhIL-21 (20 ng/ml) with IFN-α (100 ng/ml), IL-6 (100 ng/ml), IL-12 (100 ng/ml), control, or the combination of IFN-α, IL-6, and IL-12 for 7 days. Cells and culture supernatants were analyzed. (G) Representative flow plot of CD38 and CD138 expression on CD27 hi CD38 hi ASCs. Right: A summary of the percentage of CD138 + ASCs; n = 6. (H) Expression of CD11c and CD27 on CD27 − IgD − B cells. Right: Percentage of CD11c + IgD − CD27 − B cells; n = 6. (I) Expression of active-caspase-3 in B cells. Right: Percentage of active-caspase-3 + B cells from different groups; n = 3. (J) Different immunoglobulin isotype levels in the culture supernatants from (G) to (H) were measured by the multiplex assay; n = 6. Data in graphs represent mean ± SEM. Significance was tested by one-way ANOVA [(A) to (I)] and paired Student’s t test (J). [(A) to (F)] ICI, ICI control.
Techniques Used: Multiplex Assay, Clinical Proteomics, Concentration Assay, Isolation, Cell Culture, Control, Expressing
Related Articles
Injection:Article Title: Virus-specific CD4+ T cells contribute to clearance of human metapneumovirus despite exhibiting an impaired phenotype. Article Snippet: Human metapneumovirus (HMPV) is a leading cause of respiratory tract infection in pediatric, elderly, and immunocompromised populations.. Clearance of HMPV relies primarily on the destruction of infected cells by cytotoxic CD8 T cells.. However, signals provided by CD4 helper T cells significantly impact the magnitude and effectiveness of CD8 T cells. Control:Article Title: Virus-specific CD4+ T cells contribute to clearance of human metapneumovirus despite exhibiting an impaired phenotype. Article Snippet: Human metapneumovirus (HMPV) is a leading cause of respiratory tract infection in pediatric, elderly, and immunocompromised populations.. Clearance of HMPV relies primarily on the destruction of infected cells by cytotoxic CD8 T cells.. However, signals provided by CD4 helper T cells significantly impact the magnitude and effectiveness of CD8 T cells. Infection:Article Title: Virus-specific CD4+ T cells contribute to clearance of human metapneumovirus despite exhibiting an impaired phenotype. Article Snippet: Human metapneumovirus (HMPV) is a leading cause of respiratory tract infection in pediatric, elderly, and immunocompromised populations.. Clearance of HMPV relies primarily on the destruction of infected cells by cytotoxic CD8 T cells.. However, signals provided by CD4 helper T cells significantly impact the magnitude and effectiveness of CD8 T cells. other:Article Title: Germinal Center B cells provide essential IL-1β signals to TFH cells via canonical NLRP3 inflammasome activity post influenza infection Article Snippet: B cells were enriched from spleen or lymph node tissues using immunomagnetic negative selection kit (STEMCELL Technologies, Cambridge, MA). Article Title: Inflammatory arthritis irAE may represent a unique autoimmune disease primarily driven by T cells but likely not autoantibodies. Article Snippet: Briefly, cells were treated with control, 100 mM 2- deoxy- d- glucose (2- DG), 1 μM oligomycin (Oligo), or a sequential combination of the drugs at the final concentrations for 30 to 45 min; puromycin (10 μg/ml; Invitrogen) was incubated with cells for 20 min. After puromycin treatment, cells were washed in cold PBS and stained with a combination of Fc receptor blockade and cell viability dye and then primary conjugated antibodies against surface markers for 30 min on ice in FACS buffer. Article Title: Tumor-Initiating Stem Cells Fine-tune the Plasticity of Neutrophils to Sculpt a Protective Niche Article Snippet: Clinical Proteomics:Article Title: Triptolide derivative STP1 ameliorates murine systemic lupus erythematosus via targeting Fyn kinase. Article Snippet: Triptolide has demonstrated potent immunosuppressive properties in multiple autoimmune disorders, but its severe toxicity has greatly hampered clinical application.. Here, we synthesized a triptolide derivative STP1, which exhibits remarkably reduced toxicity compared with triptolide.. Immune dysfunction plays a critical role in systemic lupus erythematosus (SLE), an archetypical and refractory autoimmune disorder with limited therapeutic options and suboptimal outcomes. Cell Differentiation:Article Title: Triptolide derivative STP1 ameliorates murine systemic lupus erythematosus via targeting Fyn kinase. Article Snippet: Triptolide has demonstrated potent immunosuppressive properties in multiple autoimmune disorders, but its severe toxicity has greatly hampered clinical application.. Here, we synthesized a triptolide derivative STP1, which exhibits remarkably reduced toxicity compared with triptolide.. Immune dysfunction plays a critical role in systemic lupus erythematosus (SLE), an archetypical and refractory autoimmune disorder with limited therapeutic options and suboptimal outcomes. |
